Fig. 4. Diverse set of D-amino acids effect on biofilm formation.

S. aureus (SC01) biofilm formation was evaluated after 48 h in the presence of specified unnatural D-amino acids at a concentration of 1 mM or the positive control carvacrol (2 mM). The absorbance was recorded at 595 nm following crystal violet staining. All points are significantly different from the positive control carvacrol (****p<0.0001 relative to positive control, unpaired t-test.).