Fig 3. ADC blocks TGF-β1-induced EMT in breast cancer cells.

MCF-7 cells were pre-treated with ADC (5–20 μM) for 2 h prior to stimulation with TGF-β1 (20 ng/mL) for 48 h. (A) Morphological changes especially cell scattering was examined by phase-contrast microscope. Photomicrography shown here are from one of the three independent experiments. (B) ADC inhibits TGF-β1-induced actin cytoskeleton reorganization in MCF-7 cells. ADC and TGF-β1 treated cells were fixed, permiabilized, and stained with FITC-phalloidin to visuvalize the F-actin cytoskeleton reorganization. The images are representative of three independent experiments. Bars, 20 μm. (C) Protein samples were isolated from control, ADC, and TGF-β treated cells for the detection of E-cadherin, occluding, vimentin, N-cadherin, and β-actin proteins. β-actin was used as an internal control. Western blot data presented are representative of those obtained in at least three independent experiments.