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(A) 77 K fluorescence emission spectra of Pchlide in a water-based buffer containing imidazole. Excitation wavelength: 440nm. Pchlide concentration 0.26 μM. (B) 77 K fluorescence excitation spectra for Pchlide in a water-based buffer containing imidazole in the absence and presence of PORA. NADPH concentration: 0.2 mM. Emission wavelength: 660 nm. Pchlide concentration 0.26 μM; log[IMI:Pchlide] = 5.76.
