Fig 3. Altered PI3K signalling pathway, lipogenesis, and gluconeogenesis upon HFHS dieting.
The experiments were performed the same as described in the legend to Fig. 2. (A) Western blots of PI3Kp85, phospho-Akt (pAkt), Akt, phospho-FoxO1 (pFoxO1) and FoxO1 in the liver. (B) Western blots of phospho-Akt (pAkt), Akt, and PI3Kp85 in hypothalamus. (C) Western blots of LXRα, SREBP1c, PEPCK, phospho-GSK3α/β (pGSK3α/β), and GSK3β in the liver. § P<0.05 relative to basal (0 week). * P<0.05, ** P<0.01 between HFHS diet versus chow diet conditions.