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. 2015 Feb 6;10(2):e0117008. doi: 10.1371/journal.pone.0117008

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© 2015 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — At 0, 1, 2, 4 and 8-week feeding with chow or HFHS diet, the animals (12 h fasting) were intraperitoneally injected with insulin (0.75 U/kg body weight) or leptin (0.6 mg/kg body weight), liver samples were collected 30min after the injection. Samples were analyzed along with the non-hormone treated liver samples (A) Western blots of total and phospho-Akt (Thr³⁰⁸), PI3Kp85/p55 in the liver 30 min after insulin injection. (B) Western blots of total and phospho-STAT3, PI3K p85/p55 in the liver 30 min after leptin injection. Data (mean ± SE, n = 3) are presented as relative protein levels. ^* P<0.05, ^** P<0.01 between hormone treated versus non-hormone treated conditions.