Table 2. Liver metabolic parameters and liver enzymes.
| Parameters | Chow diet (week) | HFHS diet (week) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 4 | 8 | 1 | 2 | 4 | 8 | |
| Liver TG (mg/g) | 14.1±0.32 | 21.7±1.26 | 20.6±0.9 | 21.0±0.7 | 20.2±0.6 | 44.9±1.6** | 55.7±1.7** | 56.6±2.0** | 58.3±1.2** |
| Liver TC (mg/g) | 6.6±0.36 | 7.3±0.16 | 7.5±0.14 | 7.8±0.26 | 7.7±0.58 | 17.0±0.64** | 16.8±0.85** | 15.6±1.0** | 12.7±0.67** |
| Body weight (g) | 180±2.0 | 205±3.4 | 280±5.4 § | 317±4.2 § | 369±7.4 § | 211±3.0 | 283±3.0 | 331±5.3 † | 434±8.7 † ** |
| Liver weight (g) | 6.2±0.07 | 7.6±0.18 | 7.1±0.06 | 7. 5±0.09 | 9.9±0.07 § | 9. 9±0.26** | 14.2±0.24 † ** | 15.70±1.2 † * * | 18.7±0.40 † ** |
| Liver/body weight ratio (%) | 3.43±0.06 | 3.82±0.13 | 3.53±0.03 | 3.35±0.02 | 2.69±0.02 | 4.81±0.16 | 5.0±0.08** | 4.69±0.06** | 4.31±0.03** |
| Liver steatosis score | 0.0±0.0 | 0.1±0.01 § | 0.1±0.01 § | 0.2±0.01 § | 0.2±0.01 § | 0.2±0.01** | 0.5±0.03 † ** | 1.2±0.03 † ** | 2.2±0.06 † ** |
| Hepatocytes with lipids (%) | 0.0±0.0 | 1.2±0.02 § | 2.2±0.02 § | 2.5±0.04 § | 2.8±0.05 § | 2.6±0.14** | 4.6±0.14 † ** | 45.8±0.58 † ** | 85.9±1.00 † ** |
| ALT (u/l) | 45.6±2.4 | 36.4±1.7 | 48.3±5.5 | 52.3±3.5 | 51.6±2.8 | 42.9±1.7 | 62.5±2.4 | 52.1±1.5 | 54.4±2.7 |
| AST (u/l) | 174.3±19.4 | 61.4±1.8§ | 139.7±20.0 | 133.9±15.8 | 125.5±12.9 | 55.0±1.2 | 112.0±8.1 | 99.4±7.9 | 88.8±3.6 |
Values are means ± SE (n = 6–8 per group).
§ P < 0.05 relative to 0-week under chow diet,
† P<0.05 relative to 1-week under HFHS diet;
** P<0.01, HFHS diet versus chow diet. TG, triglyceride; TC, total cholesterol; ALT, alanine transaminase; AST, aspartate transaminase.