Fig 5. FTgpi is degraded through proteasomes.

HPL cells were transiently transfected with PrP or FTgpi, and metabolically labeled with [³⁵S]Met/Cys for 20 min at 37°C. Then, cells were either lysed after the pulse or incubated in culture medium without ³⁵S at 37°C for 1, 2, 4, and 6 h respectively, in presence of either DMSO (untreated), bafilomycin (a lysosomes inhibitor) or MG132 (a proteasomes inhibitor). Proteins were immunoprecipitated with POM2 and subjected to SDS-PAGE and autoradiography. (A) Left panel: PrP turnover was impaired upon treatment with bafilomycin but not MG132. Right panel: evaluation of autoradiograms: the amounts of protein are expressed as percentage of total protein rescued directly after the labeling period and plotted as a function of the chase time points. (B) Left panel: FTgpi turnover was impaired upon treatment with MG132 but not bafilomycin.