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. 2015 Feb 6;10(2):e0117412. doi: 10.1371/journal.pone.0117412

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© 2015 Dametto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — Brain homogenates of FTgpi155, BL6 and Prnp ^o/o mice were incubated overnight with POM2 to precipitate FTgpi and PrP. Precipitates were analyzed by Western blotting (for details see Material and Methods). (A) Right panel: total extract. Left panel: immunoprecipitates, showing that BiP specifically co-precipitated with FTgpi, as no signal was detected in Prnp ^o/o mice. (B) Right panel: total extract. Left panel: immunoprecipitates, showing that BiP does not, or minimally, co-precipitated with PrP.