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. 2015 Feb 6;10(2):e0118080. doi: 10.1371/journal.pone.0118080

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© 2015 Isobe et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) FACS analyses. Ovarian cancer ascites were collected aseptically, and cells were isolated by standard Ficoll-Paque density-gradient. Thereafter, cells were labelled with cell surface markers CD11b (PE, x-axis) and CD14 (APC, y-axis). Cells were divided into 3 groups, CD11b^-CD14^-, CD11b⁺CD14^- and CD11b⁺CD14⁺ cells. (B) The majority of CD11b⁺CD14⁺ cells are M2-polalized macrophages. These three populations of cells were further labeled with cell surface markers CD68 (Alexa Fluor-488, x-axis) and CD206 (eFluor-450, y-axis). 87.8% of CD11b⁺CD14⁺ cells (right) were CD68 and CD206 positive.