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. 2015 Feb 6;10(2):e0117655. doi: 10.1371/journal.pone.0117655

Fig 5. Characterization of RanBPM motifs that confer nuclear or cytoplasmic localization of GFP-β-gal.

Fig 5

A-G) RanBPM shRNA Hela cells were transfected with either pHM830 (830) or pHM840 (840) empty vectors (EV) or vectors containing various motifs or domains of RanBPM fused to GFP-β-gal. The identity of the RanBPM motif/domain fused to GFP-β-gal is indicated above each panel. Left, pHM830 fusion constructs, right, pHM840 fusion constructs. Cells were fixed 24 hours after transfection and nuclei stained with DAPI. GFP-β-gal subcellular localization was scored as either N>>C (completely nuclear), N>C (nuclear greater than cytoplasmic, N = C (nuclear equal to cytoplasmic), C>N (cytoplasmic greater than nuclear, or C>>N (completely cytoplasmic). Data represent averages from three separate experiments, each assessing a minimum of 50 cells. Error bars represent SD. RanBPM motifs versus EV, ***, P<0.001; **, P<0.01; *, P<0.05. Inset, representative images of transfected pHM830 or 840 fusion constructs alone (EV) or subcloned with RanBPM motifs. Scale bar: 10μm.