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. 2015 Feb 6;10(2):e0117405. doi: 10.1371/journal.pone.0117405

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© 2015 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Four weeks after injection, tumor nodules formed in the right armpits of nude mice (black arrows). (B–D) HE staining confirmed that the nodules were typical laryngeal squamous cell carcinoma containing abundant CAFs (black arrows). (E,F) Two days after seeding, the CAFs (white arrow) and HEp-2 cells (black arrow) grew out of the tissue fragments. (G,H) At passage 4, purified CAFs were obtained. (I,J) The NFs grew out of the fragment. (K,L) At passage 4, purified NFs were obtained.