Table 1. Bacterial strains and plasmids used.
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | Lab strain | |
| Bacillus | ||
| FZB42 | Wild-type isolate | BGSC 10A6 |
| E-1 | ΔclpA::Emr | This study |
| E-2 | ΔclpB::Emr | This study |
| E-3 | ΔclpC::Emr | This study |
| E-4 | ΔclpD::Emr | This study |
| Plasmids | ||
| pMD19-T | Cloning vector Ampr, lacZ＇ | Takara Co. Ltd |
| pMUTIN4 | Integration vector Emr, Ampr, lacZ＇ | BGSC ECE139 |
| pMUE-1 | pMUTIN4 containing 0.59 kb insert of clpA | This study |
| pMUE-2 | pMUTIN4 containing 0.656 kb insert of clpB | This study |
| pMUE-3 | pMUTIN4 containing 0.832 kb insert of clpC | This study |
| pMUE-4 | pMUTIN4 containing 0.872 kb insert of clpD | This study |