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. 2015 Feb 6;10(2):e0117370. doi: 10.1371/journal.pone.0117370

Fig 2. uts2b mRNA is restricted to the ventral spinal cord and hindbrainin X. laevis tadpoles.

Fig 2

Distribution of the uts2b mRNA revealed by in situ hybridization with uts2b antisense probe on whole tadpoles (A–D and H), dissected and opened CNS (E), or CNS sections (F, G) at stages 24 (A), 27 (B), 33/34 (C), 37/38 (D) 51 (E), and 55 (F, G). H. Stage 37/38 tadpole hybridized with uts2b sense probe used as negative control. A–D and H. Lateral views of tadpoles with dorsal side up and rostral to the right. E. Dorsal view of whole-mount preparation of hindbrain-spinal cord (CNS). Note that in this preparation, the more ventral structures are closer to the midline. F and G. Coronal sections at the level of the spinal cord (F) and the posterior part of brainstem (G) with dorsal up. Arrowheads point to uts2b mRNA detection in stage 24 tadpole and in F and G coronal sections. White arrows designate the region of nuclei of the trigeminal (V) nerve; asterisks denote the region of nuclei of the vagus/hypoglossal/accessory (IX–XI) nerves. r, rhombomere; V4, fourth ventricule.