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. 2015 Feb 6;10(2):e0117370. doi: 10.1371/journal.pone.0117370

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© 2015 Bougerol et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — Confocal images of combined immuno-labeling of ChAT⁺ (A1) and GFP⁺ (A2) neurons in a whole-mount spinal cord preparation of stage 50 transgenic tadpole. A3. Merged image obtained when ChAT and GFP stainings were superimposed. Dorsal view with rostral up. Only the hemi-cord is shown. The dashed-line on the right side represents the midline. Note that in this preparation, the more ventral structures are closer to the midline. Confocal images of combined immuno-labeling of ChAT⁺ (B1–C1) and GFP⁺ (B2–C2) neurons in lumbar spinal cord (B) and brainstem (C) cross-sections of stage 60 uts2b-GFP tadpoles. B3, C3. Merged images obtained when ChAT and GFP stainings were superimposed. The cross-section in C1–3 originates from the caudal hindbrain (rhombomeres 7–8) where IX-XI motor nuclei are located. Yellow arrowheads designate GFP⁺/ChAT⁺ cells; green arrowheads designate GFP⁺/ChAT⁻ cells; White arrowheads designate GFP⁻/ChAT⁺ cells. D, dorsal; M, medial; V4, 4^th ventricule.