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. 2015 Feb 6;10(2):e0117470. doi: 10.1371/journal.pone.0117470

Fig 1. Substrate specificity of MszExo I.

Fig 1

(A) MszExo I (5 nM) was incubated with 3’-overhang and 5’-overhang DNAs (50 nM) for indicated time at 37°C. The positions labeled by a 6-FAM are indicated by an asterisk for the DNA substrates. The 28-nt, 22-nt, 15-nt, 10-nt, and 5-nt poly dAs labeled by a 6-FAM at 5’-end were used as markers. Other details are described in Materials and Methods. (B) Time course experiments of the exonuclease activity of MszExo I were performed using 6-FAM labeled ssDNAs (5FAM-dA28, 5FAM-dT28 and 5FAM-dC28) as substrates. MszExo I (5 nM) was incubated with the DNA substrates (50 nM) at 37°C for indicated time. The 28-nt, 22-nt, 15-nt, 10-nt, and 5-nt poly dAs labeled by a 6-FAM at 5’-end were used as markers. Other details are described in Materials and Methods.