Fig 2. Endonuclease and RNase assay of MszExo I.

(A) MszExo I (100 nM) was incubated with circular ssDNA (M13 mp18) or dsDNA (Φ174) in the standard reaction buffer at 37°C for 1 hour. The products were analyzed by electrophoresis on a 1% agarose gel which was then stained by GoldView. (B) In the RNase assay, the 300-nt RNA internally labeled by a 6-FAM was used as the substrate. The reaction was performed at 37°C for 1 hour in the standard reaction buffer. Samples were resolved on a 6% PAGE with 7 M urea. The products were visualized by Typhoon Trio variable mode imager.