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. 2015 Feb 6;10(2):e0117470. doi: 10.1371/journal.pone.0117470

Fig 7. DSC measurements of MszExo I under different conditions.

Fig 7

(A) DSC measurements of MszExo I as a function of magnesium ions concentration. DSC measurements of MszExo I in three different buffers were performed. The base buffer was 50 mM Glycine-NaOH, 200 mM NaCl, pH 9.5. Open circle represents the base buffer in the presence of 1mM EDTA, and black line represents the best fit to two-state transition model. Open square represents the base buffer in addition of 5 mM MgCl2, and grey line represents the best fit to non-two-state transition model. Plus sign represents the base buffer in addition of 20 mM MgCl2, and red line represents the best fit to non-two-state transition model. All Tm values are shown in Table 2. (B) DSC measurements of MszExo I as a function of salt concentration. DSC measurements of MszExo I in three different buffers were performed. The base buffer was 50 mM Glycine-NaOH, 1 mM EDTA, pH 9.5. Open circle represents the base buffer, open square represents the base buffer in addition of 200 mM NaCl, and plus sign represents the base buffer in addition of 600 mM NaCl. DSC data were fitted to two-state transition model. Tm values are shown in Table 2. (C) The unfolded fraction of MszExo I monitored by the transition enthalpy. DSC Data in Fig. 7B were transformed into FU-T plots by integration of the Cp versus T curve.