Table 2. Tm value of MszExo I in different buffers.

Buffer	Tm1 (°C)	Tm2 (°C)
1 mM EDTA + B1*	44.6	No
5 mM MgCl2 + B1	53.1	57.4
20 mM MgCl2 + B1	54.8	58.4
0 mM NaCl + B2**	43.1	No
200 mM NaCl + B2	44.6	No
600 mM NaCl + B2	48.5	No

*, B1 is the buffer which is composed of 50 mM Glycine-NaOH, 200 mM NaCl, pH 9.5.

**, B2 is the buffer which is composed of 50 mM Glycine-NaOH, 1 mM EDTA, pH 9.5.