Fig 1. YhdE substrate specificity.

Two different substrate concentrations were tested. YhdE (1.5 μM) in the presence of 2 mM Mn²⁺, 20 mM HEPES buffer pH 7.0 and either 100 μM or 500 μM TTP was incubated for 10 minutes at 25°C. The percent activity for each of the different nucleotides tested is shown. Activity is expressed as a percentage of the YhdE activity using dTTP as the substrate at 500 μM. All experiments were completed in triplicate and performed twice. The error bars represent the standard error of the mean.