Fig 2. Kinetic analysis of YhdE PPase activity.

(A) UTPase activity of YhdE. For kinetic measurements, 1.5 μM YhdE was assayed under standard conditions (2 mM Mn²⁺, 20 mM HEPES buffer pH 7.0, 25°C), along with various concentrations of the substrate UTP. (B) dTTPase activity of YhdE. For kinetic measurements, 1.5 μM YhdE was assayed in standard conditions (2 mM Mn²⁺, 20 mM HEPES buffer pH 7.0, 25°C), along with various concentrations of the substrate dTTP. Kinetic parameters were calculated using SigmaPlot. All experiments were completed in triplicate and performed twice. The error bars represent the standard error of the mean.