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| Enzyme | Substrate | Kcat (min−1) | Km (mM) | Kcat/Km (mM−1 min−1) |
|---|---|---|---|---|
| PL1A | PGA (citrus) | 1.3±0.03 | 0.0313±0.0005 | 41±0.23 |
| PL1B | PGA (citrus) | 1.76±0.05 | 0.0286±0.0002 | 62±0.43 |
| PL9 | PGA (citrus) | 1.32±0.05 | 0.0378±0.0004 | 35±0.4 |
One unit of enzymatic activity (U) was defined as the amount of enzyme in mg that produces 1 mmol/L of unsaturated product per minute.
