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. 2015 Feb 6;10(2):e0115354. doi: 10.1371/journal.pone.0115354

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© 2015 Xue et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. HEK293T cells were transfected with Flag-RIG-I, Flag-MDA5, Flag-MAVS, FLAG-TBK1, Flag-IKKi, Flag-IRF3, and HA-SRSF1. Flag-tagged proteins were immunoprecipitated using anti-Flag beads and immunoblotted with the HA antibody. B. HEK293T cells were transfected with Flag-tagged cGAS, STING, or RIG-I and HA-tagged SRSF1. Flag-tagged proteins were immunoprecipitated using anti-Flag beads and immunoblotted with the anti-HA antibody. C. THP-1 cells were lysed in low-salt lysis buffer. Cell lysates were immunoprecipitated with the control antibody or the anti-RIG-I antibody, and incubated overnight with protein (A+G). Immunoprecipitated products were immunoblotted with the anti-SRSF1 antibody. WCL, whole cell lysate.