Figure 3. The association of SRSF1 with RNA polymerase III is dependent on the DNA template.

(A) HEK293T cells were transfected with salmon sperm DNA and lysed using RIPA buffer. Cell lysates were immunoprecipitated with the RNA polymerase III subunit C7 antibody and immunoblotted using the SRSF1 antibody. Lysates were left untreated or were treated for 1 h with RNase A or DNase I before immunoprecipitation. (B) HEK293T cells were stimulated with 500 ng Poly(dA:dT)/LyoVec. After 24 h, cell lysates were immunoprecipitated with the anti-RIG-I antibody or anti-Pol-III RPC32 antibody and incubated overnight with protein(A+G). Immunoprecipitated products, as well as 1% input from whole cell lysates, were immunoblotted with anti-SRSF1, anti-RIG-I, and anti-Pol III antibodies.