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. 2015 Feb 6;10(2):e0117258. doi: 10.1371/journal.pone.0117258

Fig 6. Transient over-expression of hMp84 impairs cell proliferation and stimulates cell death in HT-144 melanoma cells.

Fig 6

All parameters were evaluated at 24 h after transfection. (A) hMp84 protein (p84) was evaluated by Western blot in adhering cells of empty vector- (EV) and hMp84-transfected cells (p84); a typical Western blot out of three is shown. (B) p53 protein was evaluated by Western blot; a typical experiment out of four is shown. (C) Cell proliferation (total cell number) and (D) cell detachment (% of floating cells on total cells) were evaluated in parental, non-transfected cells (W), in EV- and in p84-cells. Cell death was evaluated as amount of intracellular LDH released in the culture medium (E), as percentage of nuclei showing an apoptotic morphology (Hoechst 33258 staining) (F), and as caspase-3/-7 enzymatic activity (G). Results are presented as mean ± S.E. of at least five independent experiments. *P<0.05; **P<0.01; ***P<0.001.