Table 1. Gene expression analysis of oxidative stress-related genes in A375 melanoma cells over-expressing hMp84.
| Gene symbol (accession number) | Description | Gene name | Fold-change |
|---|---|---|---|
| Genes involved in ROS Production/Metabolism | |||
| NCF2 (NM_000433) | Neutrophil cytosolic factor 2 | P67-PHOX | 2.54 ± 0.32 p<0.01 |
| NOX4 (NM_016931) | NADPH oxidase 4 | RENOX | 1.7 |
| SOD2 (NM_000636) | Superoxide dismutase 2, mitochondrial | MNSOD | 1.6 |
| Oxidative Stress-Responsive Genes, encoding Antioxidants | |||
| GPX2 (NM_002083) | Glutathione peroxidase 2 | GSHPx-2 | 1.9 |
| GPX3 (NM_002084) | Glutathione peroxidase 3 | GSHPx-3 | 1.5 |
| SELS (NM_203472) | Selenoprotein S | ADO15 | 1.5 |
| GCLM (NM_002061) | Glutamate-cysteine ligase, modifier subunit | GLCLR | 1.6 |
| GSTZ1 (NM_001513) | Glutathione transferase zeta 1 | GSTZ1–1 | 0.44 ± 0.05 p<0.01 |
| * SOD2 (NM_000636) | Superoxide dismutase 2, mitochondrial | MNSOD | 1.6 |
| Oxidative Stress Responsive Genes | |||
| CCL5 (NM_002985) | Chemokine (C-C motif) ligand 5 | RANTES | 2.75 ± 0.45 p<0.05 |
| DUSP1 (NM_004417) | Dual specificity phosphatase 1 | MKP-1 | 1.7 |
| FTH1 (NM_002032) | Ferritin, heavy polypeptide 1 | PIG15 | 1.5 |
| HMOX1 (NM_002133) | Heme oxygenase 1 | HO-1 | 1.5 |
| PTGS2 (NM_000963) | Prostaglandin G/H synthase and cyclooxygenase | COX-2 | 1.5 |
Gene expression was analyzed in triplicate samples by using RT2 Profiler PCR Array—Human Oxidative Stress. For NCF2, GSTZ1 and CCL5, three additional independent experiments in triplicates were performed by RT-PCR. Gene expression values in p84-cells are expressed as fold-change (mean ± S.E.) over EV-cells after 24 h of transfection.
*SOD2 has been included both in genes involved in Reactive Oxygen Species (ROS) production/metabolism and in oxidative stress-responsive genes, encoding antioxidants.