Table 1. Descriptions of reference genes in carrot (The lists of primers used in qPCR).

Gene symbol	Gene name	Arabidopsis homolog gene	Primer sequence (5’–3’) forward/reverse	Amplicon length (bp)	E (%)L/R	Tm(°C)
eIF-4α	Eukaryotic translation initiation factor 4α-1 gene	AT3G13920	TGTGCTTATCACCACTGACCTTCTG/GTCCACTACGCCCAATACGATGAA	122	108.8	82.5
ACTIN	Actin1 gene	AT2G37620	CGGTATTGTGTTGGACTCTGGTGAT/CAGCAAGGTCAAGACGGAGTATGG	98	106.2	82.5
TIP41	Tap42-interacting protein of 41 kDa gene	AT4G34270	GGAGGACTGTGAGGAACGAATTGAT/ACGCAAGAGAAGGAACCAACAACT	166	101.1	81.0
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase gene	AT1G42970	AGGCTGCTGAAGGACCATTGAAG/CCATTCGTTATCGTACCAGGCTACA	164	101.2	83.5
SAND	SAND family protein gene	AT2G28390	AATGCTGCTCACTGCTAATCCAGAT/GCCACCATCCAACATCGACCTC	124	96.8	81.0
EF-1α	Elongation factor-1αgene	AT1G07940	TCAAGGATCTCAAGCGTGGTTATGT/CAGCAATGTGGCAAGTGTGACAAT	175	100.4	84.0
PP2A	Protein phosphatase 2A gene	AT4G15415	GTGTATCAATGTACCACCAGCAACT/GCTCACCAAGGAACATGACTTCTT	147	97.3	80.0
TUB	Tubulin beta-7 gene	AT2G29550	GAGTGGAGTTACCTGCTGCCTTC/ATGTAGACGAGGGAACGGAATCAAG	94	105.5	84.0
UBQ	Polyubiquitin 10 gene	AT4G05320	TCTCCGACTCCGTGGTGGTATG/CTGCCGTCCTCCAACTGCTTAC	180	93.8	85.0