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. Author manuscript; available in PMC: 2016 Feb 4.
Published in final edited form as: Neuron. 2015 Jan 22;85(3):519–533. doi: 10.1016/j.neuron.2014.11.020

Figure 2. IL-10 increases Aβ deposition in Tg2576 mice.

Figure 2

A.8 month old Tg2576 mice were injected in the hippocampus with AAV2/1-IL-10 or AAV2/1-GFP (Control), aged for 5 months and analyzed at 13 months. Representative brain sections (cortex and hippocampus) stained with anti-Aβ mAb 33.1.1 show that Aβ plaque burden is increased in IL-10 expressing mice compared to control. No significant changes in ThioS stained cored plaques was observed. Scale Bar, 125μm (whole brain), 75μm (cortex and hippocampus). n=5-7 mice/group.

B. Quantitative burden analysis of Aβ plaque deposits show significantly increased Aβ plaque immunoreactivity but no change in ThioS reactive plaques in IL-10 expressing mice compared to controls. Data represents mean ± sem. n=5-7 mice/group. *p< 0.05, Unpaired t test.

C. Biochemical analyses of Gn-HCL solubilized Aβ42 and Aβ40 levels measured by ELISA show significantly increased insoluble Aβ42 levels in IL-10 expressing mice compared to controls but no significant differences in soluble Aβ levels (TBSx fraction) are seen. Data represents mean ± sem. n=5-7 mice/group. *p< 0.05, Unpaired t test. See also Figure S4.