. Author manuscript; available in PMC: 2016 Jan 31.

Published in final edited form as: Stem Cell Rev. 2015 Feb;11(1):87–95. doi: 10.1007/s12015-014-9542-z

Table 1.

Primers used in RT-PCR, quantitative PCR, and Net-MSP

Gene	Forward: 5′-3′	Reverse: 5′-3′	Product length (bp)
Gapdh	GGCCGCATCTTCTTGTGCAGT	TGCAAATGGCAGCCCTGGTGA	111
Cdh1	ATTCAAAGTGGCGACAGACGGC	ACCTGGGTACACGCTGGGAAACAT	223
Krt8	CAAGGTGGAACTAGAGTCCCG	CTCGTACTGGGCACGAACTTC	187
Krt18	ACTCCGCAAGGTGGTAGATGA	TCCACTTCCACAGTCAATCCA	162
Dsp	AGCCCTTTACAAGGCCATCAGCGT	TGTTCCACTGAACCAGCGTCCACA	198
Snai1	ATGCACATCCGAAGCCACACG	TGGAGCAAGGACATGCGGGAGAA	245
Snai2	CATCCTTGGGGCGTGTAAGTC	GCCCAGAGAACGTAGAATAGGTC	186
Zeb1	ACTGCAAGAAACGGTTTTCCC	GGCGAGGAACACTGAGATGT	127
Zeb2	CCACGCAGTGAGCATCGAA	CAGGTGGCAGGTCATTTTCTT	131
Fn1	GTGACACTTATGAGCGCCCTA	CCACTTGTCGCCAATCTTGTA	137
Vim	AAGCCGAAAGCACCCTGCAGTCAT	AGGTCAGGCTTGGAAACGTCCACA	202
Cdh2	ATGCCCTGAATGGAATGCTGCGGT	GCTGTGGCTGTGTTTGAAAGGCCA	211
Hes1	AGCACAGAAAGTCATCAAAGCC	ATGTCTGCCTTCTCTAGCTTGG	142
Dlx5	CACCACCCGTCTCAGGAATC	GCTTTGCCATAAGAAGCAGAGG	125
Jag1	CCTCGGGTCAGTTTGAGCTG	CCTTGAGGCACACTTTGAAGTA	150
P27^kip1	GCGGTGCCTTTAATTGGGTC	TTCGGGGAACCGTCTGAAAC	197
Isl1	ATGATGGTGGTTTACAGGCTAAC	TCGATGCTACTTCACTGCCAG	174
Lfng	GCCGTCAAGACCACCAGAAAG	GGTCATACTCCACAGCCATCTT	208
Sox2	GCGGAGTGGAAACTTTTGTCC	CGGGAAGCGTGTACTTATCCTT	157
Bmp4	CATGAGGGATCTTTACCGGCTC	TCTCCAGATGTTCTTCGTGATGG	140
Pax2	GACGAGCACCACTCTACCTG	GATGGCTGTATGGGTTGCCT	125
Myo7a	GCACTTCATCATCGGCAACGGCAT	GCTGCTCTTGGATGGGTTGTGTGT	100
Myo6	GGCATCGTCCCAAGAGATTTTC	CCACAATGTCAAAGTTCGGTACA	150
Atoh1	CCCGTCCTTCAACAACGACAAG	AGGTGATGGTGGTCATTTTTGC	156
Methylated Cdh1	GTTTTTAGTTAATTAGCGGCGTC	ACACTAAACTCGAATACGATCGAA	175
Unmethylated Cdh1	GTTTTTAGTTAATTAGTGGTGTTGG	CACTAAACTCAAATACAATCAAA	174