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. 2015 Feb 6;10(2):e0117508. doi: 10.1371/journal.pone.0117508

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© 2015 Śmiga et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — ELISA was carried out using antibodies raised against purified HmuY protein (anti-HmuY 1) and P. gingivalis wild-type (A7436 and ATCC 33277) and hmuY mutant (TO4) strains. Bacteria were cultured in high iron/heme (A) or low-iron/heme (B) media. No reactivity was observed for anti-HmuY 1–1 (epitope 1), anti-HmuY 1–2 (epitope 2), or anti-HmuY 1–3 (epitope 3) antibodies. Live bacteria were used as antigens.