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. 2015 Feb 6;10(2):e0117781. doi: 10.1371/journal.pone.0117781

Fig 1. EGFRvIII correlation with EGFR amplification and HPV.

Fig 1

A. Twenty five HNSCC tumors with known EGFR gene amplification status (via FISH) were tested for EGFRvIII positivity (28 tumors are shown, three tumors marked with N did not have gene amplification data). RNA was isolated and EGFRvIII and GAPDH were RT-PCR amplified as described in Materials and Methods. 4 of 12 EGFR amplified samples contained EGFRvIII, 5 of 12 of samples without EGFR amplification expressed EGFRvIII. All EGFRvIII bands were excised and sequenced to verify exon 1 to exon 8 joining (samples with confirmed EGFRvIII are denoted by “+”). B. A fisher’s exact test showed a lack of association between EGFRvIII expression and EGFR amplification (p = 0.56). C. A fisher’s exact test showed a lack of association between EGFRvIII expression and p16 expression (p = 0.27).