Table 4. Summary of RNA Sequencing Detection of EGFRvIII.
| Sample | Exon 1 peak coverage | Exon 8 peak coverage | Reads supporting normal | Reads supporting vIII | Fusion reads aligning to intron 1 |
|---|---|---|---|---|---|
| U87MGvIII | 597 | 1199 | 67 | 38 | 0 |
| 131 | 4 | 1 | 0 | 0 | 0 |
| 2010 | 6 | 2 | 1 | 0 | 1 |
| 21 | 4 | 3 | 3 | 0 | 0 |
| 210 | 17 | 20 | 18 | 0 | 8 |
| 21b a | 7 | 1 | 5 | 0 | 0 |
| 274 | 94 | 69 | 107 | 0 | 3 |
| 31111 | 26 | 15 | 30 | 0 | 0 |
| 35 | 20 | 4 | 7 | 0 | 1 |
| 35b b | 31 | 15 | 21 | 0 | 0 |
| 396 | 19 | 12 | 18 | 0 | 1 |
| 442 | 69 | 34 | 43 | 0 | 2 |
| 443 | 10 | 11 | 14 | 0 | 0 |
| 477 | 34 | 34 | 56 | 0 | 1 |
| 561 | 20 | 18 | 15 | 0 | 2 |
| 61 | 15 | 18 | 13 | 0 | 0 |
| 615 | 16 | 22 | 26 | 0 | 1 |
| 669 | 58 | 66 | 86 | 0 | 1 |
| 681 | 4 | 5 | 2 | 0 | 0 |
| 704 | 32 | 19 | 30 | 0 | 1 |
| 723 | 9 | 6 | 7 | 0 | 0 |
| 725 | 64 | 54 | 82 | 0 | 1 |
| 80 | 3 | 2 | 4 | 0 | 0 |
| 810 | 6 | 8 | 10 | 0 | 0 |
| 836 | 4 | 2 | 3 | 0 | 0 |
| 836b c | 2 | 2 | 0 | 0 | 0 |
a Sample 21b is a separate RNA extraction from specimen 21.
b Sample 35b is a separate RNA extraction from specimen 35.
c Sample 836b is a separate RNA extraction from specimen 836.