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. 2015 Feb 6;10(2):e0116644. doi: 10.1371/journal.pone.0116644

Fig 6. qPCR validation of selected M1 and M2a,b,c-specific genes in human GAMs.

Fig 6

We determined the expression of the M1 (IL1RN and ISG20), M2a (CLEC7A), M2b (TGFBI), and M2c-specific (CXCR4) genes in CD11b+ and CD11b- cells isolated from human GBM (CD11b+ n = 13, CD11b- n = 5), control brain (CD11b+ n = 5, CD11b- n = 2) and blood monocyte samples (n = 2). Bar graphs illustrate the absolute number of transcripts normalized to 100,000 transcripts of ACTB. Analysis was done by students t test. Error bars indicate the SEM. *, p<0.05; **, p<0.01; ***, p<0.001