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. 2015 Feb 6;10(2):e0117689. doi: 10.1371/journal.pone.0117689

Fig 2. DMSO downregulates pluripotency status in definitive endoderm-primed cells in a concentration-dependent manner.

Fig 2

(A) transcriptional and (C) flow cytometry analysis of the pluripotency marker OCT4 in definitive endoderm cells cultured for four days in the presence of 100ng/ml Activin A and varying concentrations of DMSO. (B) Euclidean-based clustering heatmap depicting qRT-PCR analysis of definitive endoderm cells for pluripotency and DE markers (low expression in green, high expression in red). The housekeeping gene GAPDH was used for normalization of the qRT-PCR results. Flow cytometry data represents 3 pooled biological replicates; qRT-PCR data is mean ± 1SD of 3 replicates, Student’s t test: n = 3, (*) p ≤ 0.05, (***) p ≤ 0.001.