Table 4. Fatty Acid Utilisation Gene mRNA Content.
| Male | Female | Stat | ||||||
|---|---|---|---|---|---|---|---|---|
| CON | HF-S | HF-FO | CON | HF-S | HF-FO | |||
| Pdk4 | EDL | 100 ± 18 | 167 ± 13* | 206 ± 25* | 131 ± 27 | 160 ± 20* | 192 ± 19* | D, M |
| SOL + | 100 ± 14 | 176 ± 5* | 234 ± 13* | 118 ± 20 | 204 ± 28* | 241 ± 24* | ||
| Ampk1 | EDL | 100 ± 7 | 97 ± 8 | 101 ± 8 | 107 ± 12 | 97 ± 6 | 128 ± 11 | M |
| SOL + | 100 ± 7 | 116 ± 7 | 118 ± 5 | 122 ± 8 | 111 ± 6 | 110 ± 6 | ||
| Ampk2 | EDL † | 100 ± 5 | 106 ± 3* | 91 ± 2 # | 85 ± 5 | 94 ± 29* | 85 ± 7 # | D MxS |
| SOL + | 100 ± 3 | 103 ± 4* | 93 ± 3 # | 95 ± 5 | 106 ± 9* | 92 ± 5 # | ||
| Acc- | EDL | 100 ± 7 | 110 ± 9 | 111 ± 10 | 140 ± 40 | 104 ± 6 | 104 ± 12 | M |
| SOL + | 100 ± 8 | 122 ± 7 | 139 ± 6 | 134 ± 28 | 147 ± 33 | 154 ± 27 | ||
| Cpt1b ‡ | EDL | 100 ± 6 | 94 ± 6 | 115 ± 4* # | 65 ± 5 | 88 ± 3 | 104 ± 10* # | DxMS |
| SOL + | 100 ± 4 | 111 ± 5 | 146 ± 4* # | 85 ± 4 | 89 ± 10 | 116 ± 9* # | ||
| Pgc1 ‡ | EDL | 100 ± 10 | 88 ± 6 | 97 ± 5 | 78 ± 6 | 79 ± 9 | 94 ± 13 | DxMS |
| SOL | 100 ± 11 ^ | 68 ± 9* | 185 ± 33* # ^ | 87 ± 10 ^ | 44 ± 7* | 144 ± 16* # ^ | ||
| Ppar | EDL | 100 ± 5 | 84 ± 6* | 90 ± 6 # | 105 ± 15 | 92 ± 11* | 95 ± 12 # | D, M |
| SOL + | 100 ± 9 | 78 ± 7* | 102 ± 7 # | 104 ± 10 | 87 ± 6* | 100 ± 8 # | ||
| Ucp3 | EDL | 100 ± 15 | 116 ± 7 | 163 ± 18* | 78 ± 14 | 105 ± 12* | 136 ± 13* | DxM |
| SOL | 100 ± 19 ^ | 196 ± 16 | 283 ± 25* | 111 ± 27 ^ | 248 ± 34* | 255 ± 24* | ||
Pyruvate dehydrogenase kinase 4 (Pdk4), AMP-activated protein kinase catalytic subunits α 1 (Ampkα 1) and α 2 (Ampkα 2), acetyl-CoA carboxylase- (Acc-), carnitine palmitoyl transferase 1b (Cpt1b), peroxisome proliferative activated receptor coactivator 1 (Pgc1), peroxisome proliferator activator receptor α (Ppar α) and uncoupling protein 3 (Ucp3) in extensor digitorum longus (EDL) and soleus (SOL) muscles of male and female mice fed control (CON), high saturated fat (HF-S) or high fat fish oil enriched (HF-FO) diets for 14 wks (Cohort 1).
mRNA contents are expressed as a percentage of the value of male animals under CON diet. Results are mean ± SEM of 9–12 animals per group. Statistics: Two-way ANOVA: Effect of diet (D):
*P≤0.05, vs CON;
# P≤0.05, compared to HF-S. Effect of muscle type (M):
+ P≤0.05 EDL vs SOL. Effect of sex (S):
‡ P≤0.05, male vs female. Muscle*sex interaction (MxS):
† P≤0.05, male vs female (of same muscle). Diet*muscle (DxM):
^P≤0.05, EDL vs SOL (of same diet).