Table 5. Abundance of Fatty Acid Utilisation and Oxidative Phosphorylation Proteins.

		Male			Female			Stat
		CON	HF-S	HF-FO	CON	HF-S	HF-FO
CPT1b	EDL	100 ± 7	74 ± 9	129 ± 19* #	104 ± 18	91 ± 25	199 ± 48* #	DxMMxS
	SOL	100 ± 22	183 ± 20*	233 ± 18*	43 ± 5 ^	141 ± 13* ^	171 ± 29* ^
PGC1α	EDL	100 ± 14	107 ± 15	107 ± 16	108 ± 16	119 ± 19	158 ± 41	M
	SOL +	100 ± 8	100 ± 9	111 ± 7	76 ± 8	82 ± 6	102 ± 10
PPARα	EDL	100 ± 16	106 ± 15	105 ± 16	99 ± 12	85 ± 7	117 ± 19	M
	SOL +	100 ± 11	78 ± 13	92 ± 16	78 ± 19	80 ± 13	87 ± 20
Complex-I	EDL	100 ± 21	77 ± 40	68 ± 21	146 ± 43	107 ± 28	88 ± 30	M
	SOL +	100 ± 18	64 ± 8	33 ± 8	49 ± 10	61 ± 16	28 ± 8
Complex-II	EDL	100 ± 10	110 ± 24	120 ± 23	136 ± 32	134 ± 22	168 ± 34	M
	SOL +	100 ± 15	94 ± 8	109 ± 7	88 ± 7	103 ± 10	105 ± 10
Complex-III	EDL	100 ± 27	31 ± 7*	68 ± 13 #	99 ± 15	35 ± 9*	94 ± 29 #	DxM
	SOL	100 ± 17 ^	60 ± 10	53 ± 11 ^	55 ± 9 ^	60 ± 15	53 ± 11 ^
Complex-IV	EDL	100 ± 17	87 ± 17	100 ± 15	106 ± 12	98 ± 13	114 ± 17	M
	SOL +	100 ± 8	104 ± 2	98 ± 8	74 ± 7	95 ± 6	101 ± 9
Complex-V	EDL	100 ± 16	106 ± 16	108 ± 15*	106 ± 11	111 ± 14	153 ± 31*	D, M
	SOL +	100 ± 14	145 ± 11	148 ± 11*	69 ± 7	130 ± 8	151 ± 10*

Carnitine palmitoyl transferase 1b (CPT1b), peroxisome proliferative activated receptor γ coactivator 1α (PGC1α), peroxisome proliferator activator receptor α (PPARα) and mitochondrial Complex-I to—V protein abundance in extensor digitorum longus (EDL) and soleus (SOL) muscles of male and female mice fed control (CON), high saturated fat (HF-S) or high fat fish oil enriched (HF-FO) diets for 14 wks (Cohort 1).

mRNA contents are expressed as a percentage of the value of male animals under CON diet. Results are mean ± SEM of 6 animals per group. Statistics: Two-way ANOVA: Effect of diet (D):

*P≤0.05, vs CON;

^# P≤0.05, compared to HF-S. Effect of muscle type (M):

⁺ P≤0.05 EDL vs SOL. Effect of sex (S):

^‡ P≤0.05, male vs female. Muscle*sex interaction (MxS):

^† P≤0.05, male vs female (of same muscle). Diet*muscle (DxM):

^P≤0.05, EDL vs SOL (of same group).