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. 2015 Feb 6;10(2):e0114813. doi: 10.1371/journal.pone.0114813

Figure 4. YAP immunofluorescent staining is non-specific in LPCs.

Figure 4

Wild-type (WT) non-transformed BMEL A-EGFP cells were stably infected with lentiviruses bearing control (Con) or YAP-targeting (KD) shRNAs. Stably infected cells were grown on coverslips and fixed before being stained with YAP (a, e, i) and counterstained with Hoechst stain (b, f, j). Overlay of YAP and Hoechst images are shown in (c, g, k). Undetectable immunofluorescence was seen with the Alexa Fluor-488 antibody alone (d, h, l).