Table 1.

Metal dissociation constants of ArgE H355A and WT ArgE determined by ITC and UV–vis absorption titration

Enzyme	Metal	Method	K d1 (μM)	K d2 (μM)
WT ArgEa	Zn(II)	ITC	2.7	51
H355A ArgE	Zn(II)	ITC	39	330
WT ArgEa	Co(II)	ITC	0.4	153
H355A ArgE	Co(II)	UV–vis Titration	1.6	NDb

^aPreviously reported (ref. (McGregor et al. 2005)).

^bNone detected.