Table 2.
Full factorial design and experimental results of exo-PG activity in the second screening step of A. sojae ATCC 20235
| Trial | Experimental factors | Response | |||
|---|---|---|---|---|---|
| Inducer substrate | Inducer concentration (%) | HCl concentration (mM) | Time (d) | exo-PG activity (U/g) | |
| 1 | Apple pomace | 10 | 0.1 | 3.5 | 28.8 |
| 2 | Sugar beet pulp | 10 | 0.1 | 3.5 | 68.2 |
| 3 | Apple pomace | 40 | 0.1 | 3.5 | 57.5 |
| 4 | Sugar beet pulp | 40 | 0.1 | 3.5 | 104.2 |
| 5 | Apple pomace | 10 | 0.22 | 3.5 | 209.5 |
| 6 | Sugar beet pulp | 10 | 0.22 | 3.5 | 300.4 |
| 7 | Apple pomace | 40 | 0.22 | 3.5 | 165.5 |
| 8 | Sugar beet pulp | 40 | 0.22 | 3.5 | - |
| 9 | Apple pomace | 10 | 0.1 | 5.5 | 4.3 |
| 10 | Sugar beet pulp | 10 | 0.1 | 5.5 | 12.5 |
| 11 | Apple pomace | 40 | 0.1 | 5.5 | 38.1 |
| 12 | Sugar beet pulp | 40 | 0.1 | 5.5 | 88.8 |
| 13 | Apple pomace | 10 | 0.22 | 5.5 | 91.7 |
| 14 | Sugar beet pulp | 10 | 0.22 | 5.5 | 374.5 |
| 15 | Apple pomace | 40 | 0.22 | 5.5 | 225.2 |
| 16 | Sugar beet pulp | 40 | 0.22 | 5.5 | - |
| 17 – 18* | Apple pomace | 25 | 0.16 | 4.5 | 118.2 ± 5.4 |
| 19 – 20* | Sugar beet pulp | 25 | 0.16 | 4.5 | 277.5 ± 1.7 |
*The standard variation between the center point repetitions was below 5%.