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Gene Expression of Crkl Neighboring Genes
Quantitative RT-PCR of genes in the LCR22B–D region flanking Crkl. Similar qRT-PCR assays were performed as in Figures 3B and 3C. Mouse hearts were isolated from Crkl-WT, Crkl-NEO/+, Crkl-NEO/NEO, and Crkl-KO hearts at E10.5. Expression values are shown as fold change and error bars are the standard deviations (FC ± SD). Statistical significance of the difference in gene expression was estimated by the two-tailed t test, p values ≤ 0.01.
