Figure 2.

Effect of HRG-β and/or t10c12 on DNA synthesis and apoptosis of HT-29 cells. HT-29 cells were cultured and treated with 4 μmol/L t10c12 in the absence or presence of 20 ng/mL HRG-β. A: To estimate DNA synthesis, [³H]thymidine incorporation into DNA was measured. B: To measure apoptosis, the cells were stained with 7-amino-actinomycin D (7-AAD) and Annexin V, and then analyzed by flow cytometry. The number of early apoptotic cells is expressed as a percentage of total cell number. Each bar represents the mean±SE from six independent experiments. Comparisons between groups that yielded significant differences are indicated by different letters above each bar. For example, ^aP<0.05 vs 0 μmol/L t10c12, ^cP<0.05 vs 0 μmol/L t10c12 + HRGβ, ^eP<0.05 vs 4 μmol/L t10c12.