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. 2014 Dec 19;13:197. doi: 10.1186/1476-511X-13-197

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© Tang; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Simultaneous treatment with arachidonic acid and linoleic acid. PC12 cells were exposed to 500 μM MPP⁺ in the absence or presence of 10 μM arachidonic acid (AA), 50 μM linoleic acid (LA) or combination of both, for 24 h at 37°C. Number of viable cells was determined by MTT assay. The control was set to 100% survival. Data are means ± SEM (n = 3). Data were analyzed using one-way ANOVA and Neuman-Keuls’ test. Means with superscripts ^a and ^b are significantly different at P < 0.05 comparing control and 500 μM MPP⁺ only-treated groups, respectively.