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. 2015 Feb 3;15:10. doi: 10.1186/s12861-015-0056-y

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© Nie et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Utilization of PIPES and HEPES result in defects in lumen morphology. (A–F) Immunofluorescence micrographs of 96 h w ¹¹¹⁸ Drosophila ommatidium in cross-section view. The rhabdomeres, F-actin, are labeled with Phalloidin (magenta) and EYS is shown in green. The tissues are fixed in (A) PEM, (B) PEM + 10% PBST, (C) PIPES, (D) HEPES, (E) PBS, (F) PBST. Scale bar, 2 μm.