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. 2014 Dec 17;15(1):1129. doi: 10.1186/1471-2164-15-1129

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© Rothwell et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transcriptional profiling of RNA-Amplified MCF7 RNA using three different protocols. Replicate samples of 25-50 pg MCF7 RNA were RNA-Amplified using three commercial kits and 5 μg of the resulting cDNA was analysed on Affymetrix arrays. (A) Miltenyi SuperAmp™ replicates showed 865 genes present across both samples with a correlation of 0.800, (B) NuGEN Ovation One-Direct™ identified 1554 with a correlation of 0.723 and (C) EpiStem RNA-Amp™ identified 2667 present with a correlation of 0.866. (D) Venn diagram showing overlap of genes present in both replicates of Miltenyi, NuGEN and EpiStem samples (all analysis based on p ≤ 0.05).