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. 2014 Dec 17;15(1):1129. doi: 10.1186/1471-2164-15-1129

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© Rothwell et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transcriptional profiling of RNA-Amplified MCF7 and MCF10A single cells. (A) Real-time PCR of RNA-Amp™ samples showing sensitive and consistent detection of 6 “housekeeping genes” in all single cell samples. (B) A heat map presentation of differentially expressed genes (LIMMA FC > 2, FDR < 0.01) detected in the Affymetrix array data from the group of 10 single cell MCF7 and MCF10A samples with blue indicating the lowest detected, red indicating the highest detected and white the midpoint. (C) PCA plot generated from the entire single cell Affymetrix array data set showing separation of all MCF7 and MCF10A samples.