Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 17;15(1):1129. doi: 10.1186/1471-2164-15-1129

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Rothwell et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

High-density real-time PCR analysis of differentially expressed gene signatures. (A) Venn diagrams showing the overlap of differentially expressed MCF7/MCF10A genes detected by high density qPCR of: unamplified cDNA from 1 μg RNA; RNA-Amp™ cDNA from 1 ng RNA; and RNA-Amp™ cDNA generated from single cells. Numbers in boxes represent the number of genes upregulated in that template type. (B) Bioinformatic analysis of the real-time PCR data identified 73 genes differentially expressed between MCF7 and MCF10A across all template types (LIMMA FC > 2, FDR < 0.01) and hierarchical clustering clearly separated the two cell lines. Heat map colour scheme for (B) as described in Figure 2B.