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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Nat Biotechnol. 2014 Dec 16;33(2):187–197. doi: 10.1038/nbt.3117

Figure 2. Sequences of off-target sites identified by GUIDE-Seq for ten RGNs.

Figure 2

For each RGN, the intended target sequence is shown in the top line with cleaved sites shown underneath and with mismatches to the on-target site shown and highlighted in color. GUIDE-Seq sequencing read counts are shown to the right of each site. The on-target site is marked with a green square and previously known off-target sites with a red diamond. Data is shown for RGNs targeting the following sites: (a) VEGFA site 1, (b) VEGFA site 2, (c) VEGFA site 3, (d) EMX1, (e) FANCF, (f) HEK293 site 1, (g) HEK293 site 2, (h) HEK293 site 3, (i) HEK293 site4, (j) RNF2. No off-target sites were found for the RGN targeted to the RNF2 site.