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. 2014 Dec 19;95(2):332–351. doi: 10.1111/mmi.12873

Figure 3.

Figure 3

Growth of L. monocytogenes strains lacking penicillin-binding proteins.A. Growth of strains lacking class A high molecular weight penicillin-binding proteins PBP A1 (Lmo1892), PBP A2 (Lmo2229) or both. Strains LMS57 (Δlmo1892), LMS64 (Δlmo2229) and LMJR30 (Ilmo1892 Δlmo2229) where cultivated in BHI broth (containing IPTG where indicated) at 37°C and optical density was measured at hourly intervals.B–D. Growth of strains lacking or conditionally expressing class B high molecular weight proteins. Strains LMJR27 (Ilmo1438, inducible PBP B1), LMJR18 (Ilmo2039, inducible PBP B2), as well as LMJR41 (Δlmo0441, PBP B3) were cultivated in BHI broth (containing 1 mM IPTG where indicated) and growth was recorded based on optical density.