Table 2.
Strains and plasmids used in this study
| Name | Relevant characteristics | Source/reference |
|---|---|---|
| Plasmids | ||
| pAUL-A | lacZα erm | (Chakraborty et al., 1992) |
| pIMK3 | Phelp-lacO lacI neo | (Monk et al., 2008) |
| pMAD | bla erm bgaB | (Arnaud et al., 2004) |
| pSH195 | Phelp-gfp neo | (Halbedel et al., 2012) |
| pUC19 | bla lacZα | Invitrogen |
| pJR4 | bla Δlmo1438 | This work |
| pJR5 | bla erm bgaB Δlmo1438 | This work |
| pJR7 | bla Δlmo2039 | This work |
| pJR8 | bla erm bgaB Δlmo2039 | This work |
| pJR10 | Phelp-lacO-lmo2039 lacI neo | This work |
| pJR17 | Phelp-lacO-lmo1438 lacI neo | This work |
| pJR18 | Phelp-lacO-lmo1892 lacI neo | This work |
| pJR20 | bla erm bgaB Δlmo2229 | This work |
| pJR24 | bla erm bgaB Δlmo0441 | This work |
| pSH239 | Phelp-gfp-lmo2229 neo | This work |
| pSH243 | erm ‘lmo2229’ | This work |
| pSH248 | Phelp-gfp-lmo1892 neo | This work |
| pSH249 | Phelp-gfp-lmo2039 neo | This work |
| pSH250 | Phelp-gfp-lmo1438 neo | This work |
| pSH251 | Phelp-gfp-lmo0441 neo | This work |
| pSH253 | bla erm bgaB 'gfp-lmo1892 | This work |
| pSH257 | bla erm bgaB Δlmo1892 | This work |
| pSH258 | Phelp-gfp-Δlmo2229 neo | This work |
| pSH277 | Phelp-gfp-Δlmo0441 neo | This work |
| pSH286 | Phelp-lacO-gfp-lmo2039 lacI neo | This work |
| L. monocytogenes strains | ||
| EGD-e | Wild type, serovar 1/2a strain | Lab collection |
| BUG1777 | ΔsrtA | (Bierne et al., 2002) |
| LMS22 | erm | (Halbedel et al., 2012) |
| LMS38 | attB::Phelp-gfp-lmo2229 neo | pSH239 → EGD-e |
| LMS44 | attB::Phelp-gfp-lmo1892 neo | pSH248 → EGD-e |
| LMS45 | attB::Phelp-gfp-lmo2039 neo | pSH249 → EGD-e |
| LMS46 | attB::Phelp-gfp-lmo1438 neo | pSH250 → EGD-e |
| LMS47 | attB::Phelp-gfp-lmo0441 neo | pSH251 → EGD-e |
| LMS57 | Δlmo1892 | pSH257 ↔ EGD-e |
| LMS64 | Δlmo2229 | pJR20 ↔ EGD-e |
| LMS141 | 'lmo2229' erm | pSH243 → EGD-e |
| LMS142 | Δlmo1892 erm | pAUL-A → LMS57 |
| LMS143 | Δlmo1892 'lmo2229' erm | pSH243 → LMS57 |
| LMJR5 | attB::Phelp-lacO-lmo2039 lacI neo | pJR10 → EGD-e |
| LMJR18 | Δlmo2039 attB::Phelp-lacO-lmo2039 lacI neo | pJR8 ↔ LMJR5 |
| LMJR20 | attB::Phelp-lacO-lmo1438 lacI neo | pJR17 → EGD-e |
| LMJR21 | Δlmo1892 attB::Phelp-lacO-lmo1892 lacI neo | pJR18 → LMS57 |
| LMJR27 | Δlmo1438 attB::Phelp-lacO-lmo1438 lacI neo | pJR5 ↔ LMJR20 |
| LMJR30 | Δlmo1892 Δlmo2229 attB::Phelp-lacO-lmo1892 lacI neo | pJR20 ↔ LMJR21 |
| LMJR41 | Δlmo0441 | pJR24 ↔ EGD-e |
The arrow (→) stands for a transformation event, and the double arrow (↔) indicates gene deletions obtained by chromosomal insertion and subsequent excision of pMAD plasmid derivatives (see Experimental procedures for details).