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Deletion of sEH attenuates UUO-elicited increases in ROS production and NADPH oxidase activity. ((a) and (b)) Three days after UUO surgery, kidneys were harvested from WT and sEH −/− mice and subjected to measurement of O2 − and H2O2 by use of HE/ethidium and DCFH-DA/DCF assays. (c) NADPH oxidase activity of kidney lysates was analyzed by NADP+/NADPH assay kit. Data are mean ± SEM from 8 mice. * P < 0.05 versus WT nonobstructed kidney; # P < 0.05 versus WT UUO kidney.
