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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Curr Biol. 2014 Jun 12;24(13):1437–1446. doi: 10.1016/j.cub.2014.05.014

Figure 4. Distribution of Dam1 molecules in the metaphase kinetochore.

Figure 4

(A) The subunit organization of the Dam1 complex [10], and the schematic of our FRET-based approach to position each subunit relative to N-Nuf2, which is proximal to the MT. (B) Proximity ratio quantification for FRET between Dam1 subunits and either N-Nuf2 or Nuf2-C. (C) Pseudo-colored Dam1 ring EM density map (based on EMDB 5254, [24]) displays the separation between equivalent points on adjacent monomers of the ring. The MT lattice is displayed in gray. (D) Proximity ratio quantification for neighboring molecules of Dam1 subunits. (E) The proximity of the tested subunits to the MT lattice and the metaphase architecture of the Dam1 complex relative to Ndc80.